What type of conjugation services do you provide?
We can help conjugate antibodies/proteins, oligo’s, small haptens, sugars and other molecules with many Flours, Lanthanide Metals & Oligo’s. Typically we help researchers conjugate either their own antibody (produced by us or themselves) or antibodies from commercial suppliers who do not provide the necessary Flour for that particular antibody. Feel free to reach out to us to see how we may help with your unique conjugation needs.
What is the Cost Recovery for you conjugation services?
This depends on many factors such as the molecule to be conjugated, quantity, concentration, subsequent purification required and the chosen Flour. For antibodies/proteins in an ideal situation where the antibody is pure, in a suitable buffer, and at a reasonable concentration conjugation services will run around $160.00 for 0.1mg of antibody.
Can you conjugate an Antibody/Protein from a Commercial Supplier?
Yes we can but there are a number of parameters that affect the ultimate outcome. Many suppliers provide antibodies at less than optimal concentrations for conjugation and many add carriers or stabilizers. In these cases often it is necessary to purify and/or concentrate the antibody/protein prior to conjugation which could lead to significant loss of the starting material. We have developed strategies to deal with these challenges but every situation can present different obstacles leading to varying outcomes. In these cases it is always best to discuss your requirements with us and determine a plan that will in the end lead to the very best chance of a successful reagent for you.
How much antibody do you require?
Again this will depend on the starting conditions of the antibody. We can successfully conjugate low ug quantities from dilute complex mixtures, however; this is not ideal and yields can be poor. Given the antibody is at a high enough concentration and in a suitable buffer we can conjugate almost as little antibody/protein as you wish to afford.
What are the ideal conditions for our antibody to be conjugated?
Your antibody/protein needs to be at a sufficiently high enough concentration ( 2mg/ml or higher) and in a buffer without contaminating primary amines or sulfhydryl groups depending on the conjugation chemistry involved. This means typically Amine or Tris buffers are not suitable as well as carrier proteins such as BSA, Gelatin or others. We do have strategies to deal with less than optimal conditions but yields can be affected depending on what is required. In such cases it is always best to discuss with us the limitations of the protein you need conjugated and how we can achieve the best reagent for you.
Do you conjugate antibody from Tissue Culture Supernatant?
Yes. We provide antibody purification services and would be happy to conjugate your antibody after purifying from tissue culture supernatant you provide or from a production run we have done for you.